The aim of present study was to improve in vitro production of bovine embryos replacing bovine serum used for in vitro maturation without compromising the subsequent development to the blastocyst stage. Influence of three macromolecular supplements was tested (fetal calf serum, bovine serum albumin and polyvinylpyrrolidone) when they were supplemented of bovine oocyte maturation medium and cultured under low O2 tension (6%). 809 cumulus-oocyte complexes, were randomly allocated in one of the three treatments groups, and each one of them was matured in the presence of a type of supplementation: a) fetal calf serum, n = 271; b) bovine albumin serum, n = 272 c) polyvinylpyrrolidone, n = 266. To evaluate the differences between groups we proceeded to count attached zygotes 48 hours postinseminción, percentage of blastocysts on days 7 and 8 postinsemination as well as total and total number of cells per blastocyst. No significant differences were found (p <0.05) in any of parameters analyzed. With these results we conclude that the bovine oocytes maturation in medium supplemented with polyvinylpyrrolidone, under low oxygen tension conditions, allows us to remove serum, without compromising the subsequent development to the blastocyst stage.