This study characterizes the bovine production system, analyzes its approximation to the organic production model, and evaluates seroprevalence of antibodies for the bovine herpes virus type 1 (BHV-1), the causal agent for Infectious Bovine Rhinotracheitis (IBR) in adult bovines without previous vaccination in 51 dairy farms in transition toward organic production in three rural production societies (RPS; Grijalva: G; Malpaso: M; Pomarrosa: P) in the municipality of Tecpatan, Chiapas, Mexico. Blood samples were taken for 213 dairy cows and 26 breeding bulls using an enzymatic immune assay (ELISA) in order to detect antibodies. The dairy farms in the three RPS make up a single production system, whose objectives, management characteristics, approximation to the organic production model (63,4%) and productivity (108,2 ± 9,4 euros of net margin per cow per year) are similar. Global seroprevalence was 62,76%, for a total of 150 samples. Seroprevalence for BHV-1 observed for dairy cows (RPS-G = 66,7%, RPS-M = 58,8%, and RPS-P = 53,8%) and that for breeding bulls (RPS-G = 100%, RPS-M = 88,9, RPS-P =66,7) did not significantly differ among the three RPS. In both cases, seroprevalence was only marginally significant for RPS-G and RPS-P. Results suggest implementation of effective IBR preventative management systems respecting the organic production guidelines.